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Related Experiment Video

Updated: Jun 4, 2026

Optimized Protocol for Intestinal Swiss Rolls and Immunofluorescent Staining of Paraffin Embedded Tissue
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Optimized Protocol for Intestinal Swiss Rolls and Immunofluorescent Staining of Paraffin Embedded Tissue

Published on: July 19, 2024

Improved protocol for processing stented porcine coronary arteries for immunostaining.

Arun H S Kumar1, Scott D McCauley, Brian G Hynes

  • 1Centre for Research in Vascular Biology (CRVB), Biosciences Institute, University College Cork, College Road, Cork, Ireland.

Journal of Molecular Histology
|February 10, 2011
PubMed
Summary

Developing improved immunostaining protocols for stented blood vessels is crucial for understanding coronary artery disease treatments. This new method enhances antibody penetration and image quality for next-generation stent development.

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Area of Science:

  • Cardiovascular Biology
  • Biomedical Engineering
  • Histopathology

Background:

  • Percutaneous coronary intervention (PCI) has transformed coronary artery disease (CAD) and myocardial infarction (MI) treatment.
  • Current stents (bare-metal and drug-eluting) have limitations like in-stent stenosis and thrombosis.
  • Understanding cellular mechanisms is vital for developing improved stent designs.

Purpose of the Study:

  • To develop an improved protocol for immunostaining stented blood vessel cross-sections.
  • To overcome technical challenges in antibody permeability and image resolution for stented vessels.
  • To facilitate detailed cellular analysis for next-generation stent development.

Main Methods:

  • Optimized resin embedding of stented porcine coronary arteries.

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Optimized Protocol for Retinal Wholemount Preparation for Imaging and Immunohistochemistry
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Optimized Protocol for Retinal Wholemount Preparation for Imaging and Immunohistochemistry

Published on: December 13, 2013

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Last Updated: Jun 4, 2026

Optimized Protocol for Intestinal Swiss Rolls and Immunofluorescent Staining of Paraffin Embedded Tissue
08:25

Optimized Protocol for Intestinal Swiss Rolls and Immunofluorescent Staining of Paraffin Embedded Tissue

Published on: July 19, 2024

Optimized Protocol for Retinal Wholemount Preparation for Imaging and Immunohistochemistry
08:32

Optimized Protocol for Retinal Wholemount Preparation for Imaging and Immunohistochemistry

Published on: December 13, 2013

  • High-speed precision diamond blade sectioning and grinding to achieve desired thickness.
  • Developed a novel deplasticization protocol for enhanced antibody permeabilization.

    • Main Results:

    • Successfully processed stented porcine coronary arteries for immunostaining.
    • Achieved improved antibody permeabilization into resin-embedded stented sections.
    • Obtained high-quality images for cellular marker analysis (smooth muscle cells, endothelial cells, monocytes, macrophages).

    Conclusions:

    • The developed protocol enables feasible and high-quality immunostaining of stented artery sections.
    • This technique aids in understanding the cellular mechanisms underlying stent performance.
    • Facilitates research towards designing and developing improved coronary stents.