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An Integrated Approach for Microprotein Identification and Sequence Analysis
Published on: July 12, 2022
Expression of non-coding RNA AB063319 derived from Rian gene during mouse development
Tiantian Gu1, Hongjuan He, Yanjiang Xing
1State Key Laboratory of Urban Water Resource and Environment, Department of Life Science and Engineering, Harbin Institute of Technology, No. 92 West Da-zhi Street, 150001, Harbin, Heilongjiang, China.
Journal of Molecular Histology
|February 10, 2011
Summary
This study investigates the long non-coding RNA AB063319 in mouse development. AB063319 shows dynamic expression, particularly in the brain and muscle, suggesting its role in organ development.
Area of Science:
- Developmental Biology
- RNA Biology
- Genomics
Background:
- Non-coding RNAs (ncRNAs) have recognized regulatory roles.
- Long intronic ncRNAs are understudied.
- The transcriptional regulation of ncRNAs in imprinted clusters remains unclear.
Purpose of the Study:
- To characterize the spatiotemporal expression of long intronic ncRNA AB063319.
- To investigate the biological functions of AB063319 during mouse development.
Main Methods:
- In situ hybridization to determine expression patterns.
- Quantitative real-time RT-PCR (QRT-PCR) for expression analysis.
- Semi-quantitative RT-PCR for postnatal expression.
Main Results:
- AB063319 is prominently expressed in the brain during early embryonic development (E10.5-E11.5).
- Expression is abundant in brain, muscle, liver, lung, and neuroendocrine tissues at E15.5.
- AB063319 expression gradually increases from E9.5 to E18.5, peaking in tongue and brain, and is also detected in postnatal day 6 (P6) tissues.
Conclusions:
- AB063319 is a stable transcriptional ncRNA.
- It likely plays crucial roles in the morphogenesis of various organs and tissues.
- Its function is particularly associated with brain and muscle development during embryonic and postnatal stages.
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