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A calcium-dependent xylan-binding domain of alkaline xylanase from alkaliphilic Bacillus sp. strain 41M-1
Risa Yazawa1, Jun Takakura, Tomoko Sakata
1Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan.
Bioscience, Biotechnology, and Biochemistry
|February 11, 2011
Abstract:
Xylanase J of alkaliphilic Bacillus sp. strain 41M-1 contains a carbohydrate-binding module family 36 xylan-binding domain (XBD). Mutational analysis of the XBD revealed that Tyr237, Asp313, Trp317, and Asp318 were involved in Ca(2+)-dependent xylan-binding, and that Asp313 and Asp318 were especially important.

