Experimental periodontal disease treatment by subgingival irrigation with tetracycline hydrochloride in rats
Related Experiment Video
Updated: Jun 4, 2026
Induction of Periodontitis via a Combination of Ligature and Lipopolysaccharide Injection in a Rat Model
Published on: February 17, 2023
Leandro Araújo Fernandes1, Thiago Marchi Martins, Juliano Milanezi de Almeida
1Department of Surgery and Integrated Clinic, Araçatuba Dental School, São Paulo State Universit, Araçatuba, SP, Brazil. leandroataunesp@ig.com.br
Objective:
The aim of this study was to compare subgingival irrigation with tetracycline hydrochloride (TTC-HCL) as adjunctive treatment to scaling and root planning (SRP) on induced periodontitis in rats.
Material And Methods:
In 60 rats, periodontal disease was ligature-induced at the mandibular left first molar. After 7 days, the ligature was removed and all animals were submitted to SRP, and divided into 2 groups according to the following treatment: C (n=30) - subgingival irrigation with 1 mL of saline; T (n=30) - subgingival irrigation with 1 mL of TTC-HCL (50 mg/mL). Ten animals in each group were euthanized at 7, 15 and 30 days posttreatment. The histometric values were statistically analyzed (p<0.05).
Results:
In the histometric analysis, at 7, 15 and 30 days, Group T (0.72 ± 0.05 mm², 0.57 ± 0.14 mm², 0.62 ± 0.07 mm²), showed less bone loss (p<0.05) than Group C (1.35 ± 0.25 mm²; 1.40 ± 0.31 mm²; 1.29 ± 0.27 mm²), respectively.
Conclusions:
Subgingival irrigation with TTC-HCL was an effective adjunctive treatment for periodontal disease induced in rats.
