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Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
Published on: January 30, 2017
Embryonic genotype and inbreeding affect preimplantation development in cattle
G Lazzari1, S Colleoni, R Duchi
1Avantea, Laboratorio di Tecnologie della Riproduzione, Via Porcellasco 7/f, 26100 Cremona, Italy. giovannalazzari@avantea.it
Reproduction (Cambridge, England)
|February 12, 2011
Summary
Purebred cattle embryos showed reduced development and delayed growth compared to crossbred embryos. Embryonic genotype and inbreeding significantly impact fertility by affecting development, gene expression, and metabolism.
Area of Science:
- Reproductive Biology
- Animal Science
- Genetics
Background:
- Cattle infertility is a significant issue with complex causes.
- Embryonic genotype and inbreeding levels are potential contributors to reproductive inefficiency.
Purpose of the Study:
- To investigate the impact of embryonic genotype and inbreeding on bovine embryo development and fertility.
- To compare cellular and molecular characteristics of purebred versus crossbred bovine embryos.
Main Methods:
- Production of purebred and crossbred bovine embryos using in vitro techniques.
- Analysis of embryo cleavage, morula/blastocyst development, amino acid metabolism, and gene expression.
- In vitro culture and subsequent in vivo transfer and recovery of embryos.
Main Results:
- Purebred embryos exhibited lower blastocyst rates and smaller size compared to crossbred embryos.
- Significant differences were observed in amino acid metabolism (serine, asparagine, methionine, tryptophan) and gene expression (MNSOD, GP130, FGF4, PLAC8).
- Purebred/inbred embryos showed reduced and delayed preimplantation development.
Conclusions:
- Embryo genotype and high inbreeding negatively affect amino acid metabolism, gene expression, and preimplantation development in cattle.
- These factors can ultimately impact overall fertility in cattle herds.
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