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Related Concept Videos

In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

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Related Experiment Video

Updated: Jun 4, 2026

Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes
11:36

Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes

Published on: January 6, 2015

In situ hybridization in flow cytometry.

V L Mosiman1, C L Goolsby

  • 1Department of Pathology, Northwestern University Medical School, Chicago, IL.

Methods in Molecular Medicine
|February 12, 2011
PubMed
Summary
This summary is machine-generated.

Molecular cytometry combines molecular techniques with flow cytometry (FCM) to better understand cellular heterogeneity in disease. This approach aids in earlier detection of infections and malignancies, and personalized cancer therapies.

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Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections
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Published on: June 25, 2021

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Last Updated: Jun 4, 2026

Simple Method for Fluorescence DNA In Situ Hybridization to Squashed Chromosomes
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Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections
07:36

Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections

Published on: June 25, 2021

Area of Science:

  • Clinical Medicine
  • Molecular Biology
  • Immunology

Background:

  • Molecular approaches are transforming disease investigation and treatment.
  • Cellular heterogeneity plays a critical role in disease processes.
  • Classical immunologic tools like flow cytometry (FCM) are essential in diagnostics.

Purpose of the Study:

  • To introduce and define molecular cytometry as a novel technique.
  • To explore the potential of combining molecular and immunologic approaches.
  • To highlight the significance of assessing cellular heterogeneity in disease.

Main Methods:

  • Integrating molecular techniques with immunologic tools, such as flow cytometry (FCM).
  • Analyzing nucleic acid sequence expression in immunophenotypically defined cell subpopulations.
  • Examining cellular heterogeneity within populations identified by gene expression.

Main Results:

  • Molecular cytometry allows exploration of gene expression in specific cell subpopulations.
  • It enables the study of cellular characteristics within genetically identified populations.
  • This integrated approach enhances the understanding of cellular heterogeneity.

Conclusions:

  • Molecular cytometry offers significant advantages for patient care.
  • Potential benefits include earlier detection of viral infections and malignancies.
  • It can improve identification of resistant cancer populations and guide targeted therapies.