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Updated: Jun 4, 2026

Mutagenesis and Analysis of Genetic Mutations in the GC-rich KISS1 Receptor Sequence Identified in Humans with Reproductive Disorders
Published on: September 4, 2011
Chemical mutational analysis of glucocorticoid receptor
1UMDS Division of Medicine, Department of Allergy and Respiratory Medicine, Guy' s Hospital, London, UK.
Abstract:
This chapter describes a chemical method for mutational analysis of the human glucocorticoid receptor (hGR) cDNA. Corticosteroid-resistant (CR) asthma is associated with in vitro and in vivo defects in mononuclear cell function (1). In addition, molecular studies using these cells have revealed that there is reduced binding of the hGR to its DNA recognition site, the glucocorticoid response element, compared to corticosteroid-sensitive (CS) controls (2). The authors therefore postulated that a point mutation of the primary structure of the GR was responsible for this functional defect, necessitating a method that would be sensitive enough to detect single-base-pair (bp) mismatches.

