High-resolution melting curve analysis to establish CYP2C19∗2 single nucleotide polymorphism: Comparison with
Manna Temesvári1, József Paulik, László Kóbori
1Chemical Research Center, Hungarian Academy of Sciences, Pusztaszeri 59-67, H-1025 Budapest, Hungary. m.temesvari@chemres.hu
Molecular and Cellular Probes
|February 15, 2011
Abstract:
Clinically significant consequences of CYP2C19 polymorphism in drug metabolism require reliable genotyping methods for assaying large numbers of blood samples. Hydrolysis single nucleotide polymorphism analysis and high-resolution melting curve (HRM) analysis successfully genotyped the 114 liver donors for CYP2C19∗2. However, the HRM analysis was found to be more efficient, rapid, user- and cost-friendly method.
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