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Related Experiment Video

Updated: Jun 4, 2026

Combining Histochemical Staining and Image Analysis to Quantify Starch in the Ovary Primordia of Sweet Cherry during Winter Dormancy
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Combining Histochemical Staining and Image Analysis to Quantify Starch in the Ovary Primordia of Sweet Cherry during Winter Dormancy

Published on: March 20, 2019

Subtle proteome differences identified between post-dormant vegetative and floral peach buds.

Constantinos Prassinos1, Stamatis Rigas, Dimosthenis Kizis

  • 1Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, Athens, Greece.

Journal of Proteomics
|February 15, 2011
PubMed
Summary

Peach trees survive winter by protecting buds. This study mapped bud proteins, revealing key roles in stress response and metabolism, with specific proteins potentially controlling sequential bud break via hydrogen peroxide signaling.

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Area of Science:

  • Plant Biology
  • Molecular Biology
  • Proteomics

Background:

  • Deciduous trees like peach require annual growth cycles for proper development.
  • Survival through winter necessitates meristem protection within floral and vegetative buds.
  • Understanding bud dormancy and break is crucial for horticultural and ecological resilience.

Purpose of the Study:

  • To elucidate the protein networks regulating bud break in peach trees.
  • To construct a reference map of the peach bud proteome.
  • To identify differentially expressed proteins between floral and vegetative buds.

Main Methods:

  • Two-dimensional gel electrophoresis (2-DE) for protein separation.
  • Protein identification and annotation using mass spectrometry.

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Establishing Pollination Requirements in Japanese Plum by Phenological Monitoring, Hand Pollinations, Fluorescence Microscopy and Molecular Genotyping
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Published on: November 9, 2020

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Last Updated: Jun 4, 2026

Combining Histochemical Staining and Image Analysis to Quantify Starch in the Ovary Primordia of Sweet Cherry during Winter Dormancy
07:25

Combining Histochemical Staining and Image Analysis to Quantify Starch in the Ovary Primordia of Sweet Cherry during Winter Dormancy

Published on: March 20, 2019

Establishing Pollination Requirements in Japanese Plum by Phenological Monitoring, Hand Pollinations, Fluorescence Microscopy and Molecular Genotyping
07:03

Establishing Pollination Requirements in Japanese Plum by Phenological Monitoring, Hand Pollinations, Fluorescence Microscopy and Molecular Genotyping

Published on: November 9, 2020

  • Comparative proteomic analysis of floral and vegetative peach buds.

    • Main Results:

    • A peach bud proteome reference map was assembled, identifying 475 proteins.
    • Most identified proteins are involved in stress response, detoxification, metabolism, and energy production.
    • Eleven proteins were differentially expressed, primarily in carbon-nitrogen homeostasis and development, including H(2)O(2)-regulating enzymes and distinct Nucleoside Diphosphate Kinase (NDPK) variants.

    Conclusions:

    • The peach bud proteome is largely conserved, with key differences enabling sequential bud break.
    • Hydrogen peroxide (H(2)O(2)) and Nucleoside Diphosphate Kinases (NDPKs) may play signaling roles in dormancy release.
    • This research provides foundational data for understanding peach bud dormancy and break mechanisms.