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Isolation of Endothelial Progenitor Cells from Healthy Volunteers and Their Migratory Potential Influenced by Serum Samples After Cardiac Surgery

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Transient extremity ischemia augments CD34+ progenitor cell availability.

David Czeiger¹, Oleg Dukhno, Amos Douvdevani

¹Soroka University Medical Center, Beer Sheva, Israel.

Stem Cell Reviews and Reports

|February 15, 2011

Summary

This summary is machine-generated.

Ischemic preconditioning safely increases stem cell yield. Brief ischemia of lower limbs significantly boosted CD34+ cell numbers, offering a promising method for enhanced stem cell availability for therapies.

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Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells

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Published on: August 9, 2019

Area of Science:

Regenerative Medicine
Hematology
Ischemic Physiology

Background:

Peripheral blood is a readily accessible source for stem cell collection.
Current methods yield relatively low numbers of stem cells, limiting therapeutic applications.
The potential of ischemic preconditioning to enhance stem cell mobilization remains largely unexplored.

Purpose of the Study:

To investigate the safety and efficacy of brief, transient lower limb ischemia.
To determine if ischemic preconditioning can augment the number of harvestable CD34+ cells from peripheral blood.
To assess the correlation between stem cell increase and specific cytokine concentrations (IL-8, VEGF).

Main Methods:

Healthy volunteers underwent brief, transient ischemia of the lower limbs.
Peripheral blood samples were collected at baseline, 30 minutes, 12 hours, and 24 hours post-ischemia.
Quantification of total progenitor cells and CD34+ cells via flow cytometry after 5-day culture; IL-8 and VEGF levels were measured.

Main Results:

No serious adverse events were reported, indicating a safe procedure.
A significant increase in total cells (from 0.46 to 0.7 × 10^6 cells/ml) and CD34+ cells (from 4.23 to 7.17 × 10^4 cells/ml) was observed 12 hours post-ischemia (p < 0.003 and p < 0.0001, respectively).
The increase in CD34+ cells correlated positively with IL-8 levels but not VEGF.

Conclusions:

Ischemic preconditioning is a safe and effective method to increase peripheral blood stem cell yield.
This technique enhances the availability of CD34+ cells for potential therapeutic applications.
The observed stem cell augmentation is linked to elevated IL-8 concentrations.