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Related Concept Videos

In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

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Related Experiment Video

Updated: Jun 4, 2026

Double Fluorescence in situ Hybridization in Fresh Brain Sections
12:15

Double Fluorescence in situ Hybridization in Fresh Brain Sections

Published on: August 14, 2010

In situ hybridization on brain tissue.

C H Lammers, Y Hara, M Maral Mouradian

    Methods in Molecular Medicine
    |February 15, 2011
    PubMed
    Summary

    In situ hybridization (ISH) visualizes messenger RNA (mRNA) distribution in cells and tissues. This technique allows for precise localization of transcripts and quantitative analysis of gene expression changes.

    Area of Science:

    • Molecular Biology
    • Neuroscience
    • Genetics

    Background:

    • In situ hybridization (ISH) is a key technique for analyzing gene expression.
    • It allows for the visualization of specific nucleic acid sequences within their cellular or tissue context.

    Purpose of the Study:

    • To describe the utility of in situ hybridization (ISH) for determining mRNA distribution.
    • To highlight ISH's role in localizing transcripts and comparing gene and protein expression.
    • To emphasize ISH as a tool for quantitative gene expression analysis.

    Main Methods:

    • Hybridization of nucleic acid probes (DNA or RNA) with target nucleic acids (mRNA).
    • Application of ISH in cells, tissue preparations, and whole organisms.
    • Utilizing ISH for neuroanatomic comparisons and analysis of experimental conditions.

    More Related Videos

    Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization
    11:24

    Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization

    Published on: June 17, 2015

    Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections
    07:36

    Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections

    Published on: June 25, 2021

    Related Experiment Videos

    Last Updated: Jun 4, 2026

    Double Fluorescence in situ Hybridization in Fresh Brain Sections
    12:15

    Double Fluorescence in situ Hybridization in Fresh Brain Sections

    Published on: August 14, 2010

    Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization
    11:24

    Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization

    Published on: June 17, 2015

    Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections
    07:36

    Combining Multiplex Fluorescence In Situ Hybridization with Fluorescent Immunohistochemistry on Fresh Frozen or Fixed Mouse Brain Sections

    Published on: June 25, 2021

    Main Results:

    • ISH enables precise localization of mRNA transcripts within cells, tissues, and whole bodies.
    • It facilitates neuroanatomic comparisons between specific mRNA expression and corresponding protein levels.
    • ISH serves as a method to detect quantitative changes in gene expression across different neuroanatomic regions.

    Conclusions:

    • In situ hybridization is a versatile method for mapping gene expression at the cellular and tissue level.
    • ISH provides insights into the spatial relationship between mRNA and protein expression.
    • The technique is valuable for studying gene expression dynamics under various experimental settings.