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Updated: Jun 4, 2026

Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation

Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation

Published on: July 24, 2013

Embryo manipulation after mid-gestation stages in mice.

T Hatta¹, H Naora, J Udagawa

¹Assistant Professor of Anatomy, Department of Anatomy, Shimane Medical University, Izumo, Japan.

Surgical Technology International

|February 15, 2011

Summary

This study introduces a novel technique combining mouse embryo manipulation with ex utero development. This method aids in studying developmental biology and evaluating fetal surgery techniques for congenital disorders.

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Area of Science:

Developmental Biology
Experimental Embryology
Surgical Innovation

Background:

Direct manipulation of mammalian embryos is crucial for understanding development and assessing fetal surgery.
Ex utero mouse development, pioneered by Muneoka et al., is a valuable technique in experimental embryology.

Purpose of the Study:

To establish experimental animal models for congenital disorders using ex utero development.
To investigate the mechanisms underlying normal and abnormal morphogenesis.
To present a combined technique of ex utero development and microinjection in mouse embryos.

Main Methods:

Application of the ex utero mouse development technique.
Integration of a microinjection system with ex utero embryo manipulation.

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Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation

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Main Results:

Successful establishment of experimental animal models for congenital disorders.
Examination of mechanisms governing normal and abnormal embryonic development.
Demonstration of a refined technique for advanced embryological research.

Conclusions:

The combined ex utero development and microinjection technique offers a powerful tool for studying mammalian embryogenesis.
This method facilitates research into congenital disorders and the development of prenatal surgical interventions.