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Evaluation of acetone as organic modifier in SPE for bioanalytical quantitative LC-MS/MS
Robert MacNeill1, Esther Evans
1Department of Bioanalytical Services, Huntingdon Life Sciences, Mettlers Road, East Millstone, NJ 08875-2360, USA. macneillr@princeton.huntingdon.com
Background:
To assess the suitability of acetone as an alternative to acetonitrile in SPE under otherwise commonly used conditions, with a focus on selectivity with regard to the most abundant phospholipid class. Two representative analytes were included, leuprolide and tramadol, a peptide and a small molecule, respectively.
Results:
The use of acetone resulted in analogous elution profiles of all monitored compounds in the majority of conditions. The only significant difference was on silica-based C18, where acetone effected markedly enhanced elution of lysophosphatidylcholines than did acetonitrile. Unmodified silica was shown to operate in per aqueous LC mode in highly aqueous conditions.
Conclusion:
Acetone was established as a selectively similar and slightly more eluotropic alternative to acetonitrile in bioanalytical SPE.
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