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Updated: Jun 4, 2026

In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays
Published on: December 29, 2021
Differential functional properties of chromosomal- and plasmid-encoded H-NS proteins
Rosa C Baños1, Sonia Aznar, C Madrid
1Institut de Bioenginyeria de Catalunya, Baldiri Reixac 15-21, 08028 Barcelona, Spain.
Abstract:
The nucleoid-associated protein H-NS can be either chromosomal- or plasmid-encoded. We provide in this report evidence indicating that chromosomal- and plasmid-encoded H-NS proteins may differ in their functional properties. The modulatory function of chromosomal H-NS is antagonized by the H-NST(EPEC) protein. We show that the H-NS protein encoded by the IncHI plasmid R27 (H-NS(R27)) is less sensitive to H-NST(EPEC) antagonism than its chromosomal counterpart. H-NS(R27) plays a relevant role by modulating R27 conjugation in response to temperature. Hence, we suggest that this modulator has evolved to avoid the deregulation of R27 conjugation by H-NST(EPEC)-like proteins.
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