On-chip multi-electrochemical sensor array platform for simultaneous screening of nitric oxide and peroxynitrite
Damien Quinton1, Aurélie Girard, Loan To Thi Kim
1Unité de Pharmacologie Chimique et Génétique et Imagerie, CNRS 8151, École Nationale Supérieure de Chimie de Paris, Chimie ParisTech, Université Paris Descartes, Paris, France.
Lab on a Chip
|February 16, 2011
Summary
This study presents a novel electrochemical sensor array for simultaneous detection of nitric oxide (NO) and peroxynitrite (ONOO(-)). The device enables real-time monitoring of these crucial biological signaling molecules.
Area of Science:
- Electrochemistry
- Biosensors
- Analytical Chemistry
Background:
- Nitric oxide (NO) and peroxynitrite (ONOO(-)) are key biological signaling molecules.
- Simultaneous detection of NO and ONOO(-) is challenging but crucial for understanding biological processes.
Purpose of the Study:
- To design and fabricate a novel electrochemical sensor array (ESA) for simultaneous amperometric detection of NO and ONOO(-).
- To evaluate the analytical performance and selectivity of the developed ESA.
Main Methods:
- Microfabrication of an ESA with individually addressable gold ultramicroelectrodes (UMEs).
- Electrochemical modification of UMEs for NO detection (poly(eugenol) and poly(phenol) layers).
- Amperometric detection of NO at 0.8 V vs. Ag/AgCl and ONOO(-) at -0.1 V vs. Ag/AgCl.
Main Results:
- Successful design and microfabrication of the ESA.
- Demonstrated simultaneous amperometric detection of NO and ONOO(-) in phosphate buffer solution (PBS).
- Evaluated selectivity against common biological interferents.
Conclusions:
- The developed ESA allows for the first time simultaneous detection of NO and ONOO(-).
- The sensor array shows promise for real-time monitoring of these biologically relevant molecules in physiological conditions.
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