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Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets

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Droplet size based separation by deterministic lateral displacement-separating droplets by cell--induced shrinking.

Haakan N Joensson¹, Mathias Uhlén, Helene Andersson Svahn

¹Div of Nanobiotechnology, Royal Institute of Technology, KTH, Albanova University Center, SE-10691 Stockholm, Sweden. hakan.jonsson@biotech.kth.se

|February 16, 2011

Summary

We developed a novel microfluidic method for high-throughput droplet size separation using deterministic lateral displacement (DLD). This technique successfully sorts shrunken yeast-containing droplets from larger media droplets, enabling applications like clonal selection.

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Area of Science:

Biotechnology
Microfluidics
Cell Biology

Background:

Microfluidic droplet manipulation is crucial for high-throughput biological assays.
Separating droplets based on content properties requires precise control over droplet size and physical characteristics.

Purpose of the Study:

To present a novel method for passive, high-throughput microfluidic droplet separation by size using deterministic lateral displacement (DLD).
To demonstrate the separation of droplets based on biological content-induced physical property changes, specifically cell shrinkage.

Main Methods:

Utilized deterministic lateral displacement (DLD) for passive droplet size separation.
Incubated droplets containing Saccharomyces cerevisiae and observed size changes compared to control droplets with only yeast media.
Demonstrated sorting of shrunken yeast-containing droplets from larger media-only droplets.

Main Results:

Droplets with Saccharomyces cerevisiae significantly shrink during incubation, while media-only droplets maintain or increase size.
The DLD device successfully sorted shrunken yeast-cell droplets from 31% larger media droplets present in a >40-fold excess.
Achieved separation of 11 µm from 30 µm droplets at a rate of 12,000 droplets per second.

Conclusions:

Deterministic lateral displacement (DLD) offers high-throughput, size-based droplet separation.
This method enables droplet separation based on biological properties inferred from physical changes, such as cell shrinkage.
The demonstrated technique shows potential for applications like clonal selection in high-throughput screening.