Related Experiment Video
Updated: Jun 4, 2026

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy
Published on: June 28, 2016
Two-photon excited hemoglobin fluorescence
Abstract:
We discovered that hemoglobin emits high energy Soret fluorescence when two-photon excited by the visible femtosecond light sources. The unique spectral and temporal characteristics of hemoglobin fluorescence were measured by using a time-resolved spectroscopic detection system. The high energy Soret fluorescence of hemoglobin shows the spectral peak at 438 nm with extremely short lifetime. This discovery enables two-photon excitation fluorescence microscopy to become a potentially powerful tool for in vivo label-free imaging of blood cells and vessels.
Related Concept Videos
Photoluminescence: Fluorescence and Phosphorescence
A pair of electrons in a...
Fluorescence and Phosphorescence: Instrumentation

