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High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

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Published on: June 28, 2016

Two-photon excited hemoglobin fluorescence.

Wei Zheng, Dong Li, Yan Zeng

Biomedical Optics Express

|February 18, 2011

Summary

This summary is machine-generated.

Hemoglobin emits high-energy Soret fluorescence when excited by visible light. This discovery advances two-photon microscopy for label-free imaging of blood cells and vessels.

Keywords:

(170.0170) Medical optics and biotechnology (170.2520) Fluorescence microscopy (180.4315) Nonlinear microscopy (300.6410) Spectroscopy, multiphoton (300.6500) spectroscopy, time-resolved

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Area of Science:

Biophysics
Optical Imaging
Spectroscopy

Background:

Hemoglobin is the primary protein in red blood cells, responsible for oxygen transport.
Two-photon excitation microscopy offers advantages for deep tissue imaging due to reduced photobleaching and scattering.

Purpose of the Study:

To investigate the fluorescence properties of hemoglobin under two-photon excitation.
To explore the potential of hemoglobin fluorescence for biological imaging applications.

Main Methods:

Utilizing visible femtosecond laser sources for two-photon excitation of hemoglobin.
Employing a time-resolved spectroscopic detection system to measure fluorescence characteristics.

Main Results:

Hemoglobin exhibits high-energy Soret fluorescence upon two-photon excitation.
The fluorescence spectrum peaks at 438 nm with a very short lifetime.
Observed unique spectral and temporal characteristics of hemoglobin fluorescence.

Conclusions:

Two-photon excited hemoglobin fluorescence is a viable phenomenon.
This finding establishes two-photon excitation fluorescence microscopy as a promising tool for in vivo, label-free imaging of blood cells and vessels.