Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

1.2K
At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
1.2K
RNA Structure01:23

RNA Structure

78.5K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
78.5K
RNA Structure01:19

RNA Structure

6.6K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
6.6K
Ribosome Profiling02:24

Ribosome Profiling

4.0K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.0K
RNA Stability01:53

RNA Stability

35.5K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
35.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Towards time-resolved MicroED grid preparation using mix-and-inject gas dynamic virtual nozzles.

IUCrJ·2026
Same author

Nucleotide-Specific RNA Conformations and Dynamics as Precursors to Ribonucleoprotein Condensates.

Journal of the American Chemical Society·2025
Same author

Advances in microfluidic mixers for time-resolved structural biology with X-rays.

Biophysical reviews·2025
Same author

Nucleotide-specific RNA conformations and dynamics within ribonucleoprotein condensates.

bioRxiv : the preprint server for biology·2025
Same author

Implementation of simultaneous ultraviolet/visible and x-ray absorption spectroscopy with microfluidics.

The Review of scientific instruments·2025
Same author

Sequence-dependent conformational preferences of disordered single-stranded RNA.

Cell reports. Physical science·2024

Related Experiment Videos

Time resolved SAXS and RNA folding.

Lois Pollack1

  • 1School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA. lp26@cornell.edu

Biopolymers
|February 18, 2011
PubMed
Summary
This summary is machine-generated.

Time-resolved Small-angle X-ray scattering (SAXS) reveals early RNA folding events. This technique tracks conformational changes and transient structures during magnesium-ion-induced folding, offering unique insights into RNA dynamics.

Related Experiment Videos

Area of Science:

  • Biophysics
  • Structural Biology
  • Biochemistry

Background:

  • Small-angle X-ray scattering (SAXS) offers low-resolution structural insights into macromolecules in solution.
  • Understanding RNA folding pathways is crucial for deciphering biological function.

Purpose of the Study:

  • To investigate the time-dependent conformational changes of RNA during magnesium-ion-induced folding.
  • To characterize transient structural intermediates populated during the early stages of RNA folding.

Main Methods:

  • Coupling rapid mixing techniques with time-resolved Small-angle X-ray scattering (SAXS).
  • Monitoring global structural changes of RNA in solution upon addition of Mg(2+).

Main Results:

  • SAXS successfully captured dynamic, time-dependent structural rearrangements of RNA.
  • The study identified and characterized transient structural intermediates during the Mg(2+)-triggered folding process.
  • Early folding events, often missed by other methods, were detected.

Conclusions:

  • Time-resolved SAXS is a powerful tool for studying the dynamics of RNA folding.
  • This method provides unique insights into the global structures of transient intermediates during folding.
  • SAXS can reveal crucial early events in macromolecular folding pathways.