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Updated: Jun 4, 2026
Evaluating the Effectiveness of Cancer Drug Sensitization In Vitro and In Vivo
Published on: February 6, 2015
[Cisplatin-induced apoptosis in human lung adenocarcinoma cell line A549]
Wenzhe Liu1, Jinhan Li, Yide Hu
1Department of Oncology, Guangdong 177 Hospital, Guangzhou, Guangdong 510317, P.R.China.
Background:
To elucidate the pattern and mechanism of cisplatin-induced apoptosis and its role in tumor chemotherapy.
Methods:
Apoptosis induced by cisplatin in human lung adenocarcinoma cell line A549 was detected by cell morphology, agarose gel electrophoresis, DNA-end-labeling and flow cytometry analysis techniques.
Results:
Cisplatin-induced apoptosis of A549 cells persisted and augmented gradually from 12 to 72 hours after treated with 3 mg/L cisplatin. All of A549 cells treated respectively with 1, 3, 5 and 7 mg/L cisplatin showed apoptosis. Apoptotic effects increased in a time-dependent pattern and a concentration-dependent pattern. A549 cells were blocked in G1 phase after treated with cisplatin.
Conclusions:
Induction of cell apoptosis may be an important mechanism of anti-tumor efficacy of cisplatin.