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Semiautomated preparation of DNA templates for large-scale sequencing projects
V Smith1, C M Brown, A T Bankier
1Medical Research Council Laboratory of Molecular Biology, Cambridge.
DNA Sequence : the Journal of DNA Sequencing and Mapping
|January 1, 1990
Abstract:
The rate limiting step in a large-scale sequencing project is the generation of single-stranded DNA templates. We describe a fast, semiautomated procedure, using 96-well microtitre plates, in which 192 templates can be readily prepared in 1 day. The technique can be carried out manually or can be semiautomated using a robot pipetting device. We also provide evidence for the reliability and applicability of this method to a large-scale sequencing project.