Related Experiment Video
Updated: Jun 4, 2026
Accessing the Cytotoxicity and Cell Response to Biomaterials
Published on: July 8, 2021
Cytotoxicity comparison of mineral trioxide aggregates and EndoSequence bioceramic root repair materials
Beth Ann Damas1, Michelle A Wheater, Josef S Bringas
1Department of Endodontics, University of Detroit Mercy, Detroit, Michigan 48208-2576, USA. bethann.dds@gmail.com
Introduction:
The purpose of this bench top evidence level 5 in vitro study was to compare the cytotoxic effect of 2 brands of white mineral trioxide aggregate cement (ProRoot MTA and MTA-Angelus), Brasseler EndoSequence Root Repair Material, and Brasseler EndoSequence Root Repair Putty by using human dermal fibroblasts.
Methods:
The cells were cultured in recommended culture conditions and exposed to the tested materials. The cytotoxic effects were recorded at an observation period of 24 hours by using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-based colorimetric assay. Results were analyzed by using one-way analysis of variance with significance of p < .05.
Results:
All materials tested demonstrated cell viability ≥ 91.8%. Overall, there was no statistically significant difference in cell viability of ProRoot MTA, MTA-Angelus, and Brasseler EndoSequence Root Repair Material. However, there was a statistically significant difference negatively associated with the cell viability of human dermal fibroblasts in association with the Brasseler EndoSequence Root Repair Putty.
Conclusions:
The Brasseler EndoSequence Root Repair Materials were shown to have similar cytotoxicity levels to those of ProRoot MTA and MTA-Angelus.