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Updated: Jun 4, 2026

Estimating the Yield of Compounds on the TLC Plate via the Blue-LED Illumination Technique
Published on: October 6, 2022
Validation of thin layer and high performance thin layer chromatographic methods
B Renger1, Z Végh, K Ferenczi-Fodor
1Bernd Renger Consulting, Fritz-Reichle-Ring 2, 78315 Radolfzell, Germany. bernd.renger@br-consult.eu
Abstract:
Analytical validation is a key requirement to asses and to prove a method's reliability and suitability for an intended use. Planar chromatographic procedures are used in different applications ranging from simple screening tests to sophisticated instrumental quantitative assays of analytes in complex matrices. This paper intends to give guidance on how to adopt international accepted formal requirements and guidelines for validation of these different TLC/HPTLC procedures. In addition, some selected parameters for robustness testing and for on going quality assurance of analytical performance based on control charts are reported.
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