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Tissue-engineered larynx using perfusion-decellularized technique and mesenchymal stem cells in a rabbit model.
Nan Hou1, Pengcheng Cui, Jiasheng Luo
1Department of Otorhinolaryngology Head and Neck Surgery, First Affiliated Hospital, Chengdu Medical College, Sichuan Province, PR China. bihaichaoxin.hn@163.com
Acta Oto-Laryngologica
|February 22, 2011
Summary
Tissue-engineered larynx construction is feasible using decellularized laryngeal matrix and mesenchymal stem cells (MSCs). In vivo maturation in the omentum is a key step for future laryngeal reconstruction.
Area of Science:
- Regenerative Medicine
- Biomaterials Science
- Tissue Engineering
Background:
- Extracellular matrix (ECM) and mesenchymal stem cells (MSCs) are crucial for organ reconstruction.
- Developing functional tissue-engineered organs requires suitable scaffolds and cell sources.
Purpose of the Study:
- To create a decellularized laryngeal scaffold with intact cartilage using perfusion-decellularization.
- To recellularize the scaffold with MSCs and evaluate larynx construct development.
Main Methods:
- Rabbit larynges underwent perfusion-decellularization using detergents.
- Scaffolds were reseeded with induced MSCs and matured in rabbit omentums for 4-8 weeks.
- Evaluations included macroscopic visualization, histology, SEM, and immunohistochemistry.
Main Results:
- Perfusion-decellularization effectively removed cells while preserving ECM structure and collagen fibers.
- High chondrocyte vitality was maintained.
- Significant vascularization and integrated cartilage frameworks were observed by 8 weeks, with muscle bundles and vessels evident.
Conclusions:
- Reseeding MSCs into decellularized laryngeal matrix is a feasible approach for tissue-engineered larynx construction.
- In vivo omentum maturation is a viable strategy for pre-implantation conditioning.
- This method offers a promising avenue for tissue-engineered laryngeal reconstruction.
