Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...

Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

Immunofluorescence Microscopy

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...

Immunoprecipitation

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

CD2-induced apoptosis in activated human peripheral T cells: a Fas-independent pathway that requires early protein tyrosine phosphorylation.

Journal of immunology (Baltimore, Md. : 1950)·1996

Same author

gamma-c gene transfer into SCID X1 patients' B-cell lines restores normal high-affinity interleukin-2 receptor expression and function.

Blood·1996

Same author

Clinical, hematologic, and biochemical features of a syndrome in Bernese mountain dogs characterized by hepatocerebellar degeneration.

Journal of the American Veterinary Medical Association·1996

Same author

A phase II trial of partially incompatible bone marrow transplantation for high-risk acute lymphoblastic leukaemia in children: prevention of graft rejection with anti-LFA-1 and anti-CD2 antibodies. Société Française de Greffe de Moelle Osseuse.

British journal of haematology·1996

Same author

Radiommunological measurement of leptin in plasma of obese and diabetic human subjects.

Endocrinology·1996

Same author

Basic innervation pattern and distribution of classic autonomic neurotransmitters in human nasal mucosal vasculature.

The Laryngoscope·1996

Search research articles

Related Experiment Video

Updated: Jun 4, 2026

Histological-Based Stainings Using Free-Floating Tissue Sections

Histological-Based Stainings Using Free-Floating Tissue Sections

Published on: August 25, 2020

Immunocytochemistry and immunohistochemistry.

J Springer¹, A Fischer

¹Allergy Research Group, Humboldt University Charité, Berlin, Germany.

Methods in Molecular Medicine

|February 22, 2011

Summary

Immunohistochemistry enables precise localization of biological substances, including inflammatory markers and their receptors. This technique allows simultaneous detection of ligands and receptors using various antibody types and double-labeling protocols.

Area of Science:

Biochemistry
Immunology
Cell Biology

Background:

Immunohistochemistry is a key technique for localizing organic substances within tissues.
It is particularly useful for studying inflammatory markers, cytokines, and their receptors.

Purpose of the Study:

To highlight the utility of immunohistochemistry for localizing diverse biomolecules.
To discuss methods for simultaneous detection of ligands and receptors.
To explore antibody choices for immunohistochemical applications.

Main Methods:

Utilizing immunohistochemistry for histochemical localization.
Employing double-labeling protocols for simultaneous detection of ligands and receptors.
Selecting monoclonal or polyclonal antibodies against purified proteins or synthetic peptides.

More Related Videos

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections

Published on: November 14, 2016

Immunocytochemistry: Human Neural Stem Cells

Immunocytochemistry: Human Neural Stem Cells

Published on: August 24, 2007

Related Experiment Videos

Last Updated: Jun 4, 2026

Histological-Based Stainings Using Free-Floating Tissue Sections

Histological-Based Stainings Using Free-Floating Tissue Sections

Published on: August 25, 2020

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections

Published on: November 14, 2016

Immunocytochemistry: Human Neural Stem Cells

Immunocytochemistry: Human Neural Stem Cells

Published on: August 24, 2007

Main Results:

Demonstration of inflammatory markers, biogenic amines, cytokines, and receptors is possible.
Simultaneous detection of corresponding ligands and receptors is feasible with compatible protocols.
Synthetic peptides can generate site-specific antisera for receptor subtype analysis.

Conclusions:

Immunohistochemistry is a versatile method for precise molecular localization in tissues.
Double-labeling protocols enhance the ability to study ligand-receptor interactions.
Antibody selection, including those against synthetic peptides, impacts specificity and application.