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Related Experiment Video

Updated: Jun 4, 2026

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics
10:50

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

Published on: July 16, 2018

Electrically sensing protease activity with nanopores.

Mikiembo Kukwikila1, Stefan Howorka

  • 1Department of Chemistry, University College London, London WC1H 0AJ, UK.

Journal of Physics. Condensed Matter : an Institute of Physics Journal
|February 23, 2011
PubMed
Summary
This summary is machine-generated.

Enzymatic activity was detected electrically using nanopore recordings. This simple method, sensing protease activity via ionic current blockades, may be applicable to other enzymes.

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Last Updated: Jun 4, 2026

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics
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Published on: December 2, 2011

Area of Science:

  • Biochemistry
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Protease activity is crucial in biological processes.
  • Sensing enzymatic activity often requires complex assays.
  • Nanopore technology offers a novel platform for biological detection.

Purpose of the Study:

  • To develop a simple electrical method for detecting protease enzymatic activity.
  • To demonstrate the feasibility of using nanopore recordings for enzyme sensing.
  • To explore the potential application of this method for various proteases.

Main Methods:

  • Peptide substrates were immobilized on microscale beads.
  • Enzymatic cleavage by trypsin released soluble peptide fragments.
  • Fragments were driven through an α-hemolysin protein pore via electrophoresis.
  • Ionic current blockades were recorded to detect enzymatic activity.

Main Results:

  • Electrical detection of protease activity was achieved using nanopore recordings.
  • Cleavage of tethered peptide substrates by trypsin generated detectable ionic current changes.
  • The method demonstrated sensitivity to enzymatic activity.

Conclusions:

  • A straightforward electrical detection method for protease activity using nanopore recordings was established.
  • This technique offers a simple and potentially versatile approach for sensing enzymatic activity.
  • The method holds promise for broader applications in enzyme detection and analysis.