Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Raman Spectroscopy Instrumentation: Overview

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Overcoming stress limitations in SiN nonlinear photonics via a bilayer waveguide.

Nanophotonics (Berlin, Germany)·2025

Same author

Simultaneous on-chip generation of violet, blue, cyan, green, yellow, orange, and red light from an octave-spanning infrared frequency comb.

Optics express·2025

Same author

Nonlinear Dynamics of Coupled-Resonator Kerr Combs.

Physical review letters·2025

Same author

Automated tuning of a ring-assisted MZI-based interleaver for Kerr frequency combs.

Optics letters·2025

Same author

Clearing a path for light through non-Hermitian media.

Nanophotonics (Berlin, Germany)·2024

Same author

Influence of discontinuities on photonic waveguides.

Optics letters·2024

Same journal

Generalizable framework for multi-site bone density prediction using non-dominant wrist optical biomarkers.

Biomedical optics express·2026

Same journal

Erratum: Review of dynamic optical coherence tomography for intracellular motility [Invited]: errata.

Biomedical optics express·2026

Same journal

Digital-micromirror-device-based illumination strategies for background suppression in single-molecule localization microscopy.

Biomedical optics express·2026

Same journal

Synergistic combination of convective self-assembly and hollow core fiber for sensitive SERS detection of glucose molecules.

Biomedical optics express·2026

Same journal

Multimodal diagnostic network integrating infrared and mass spectra for lung cancer.

Biomedical optics express·2026

Same journal

Multimodal Optical Biosensing for Precision Medicine and Healthcare: Introduction to the feature issue.

Biomedical optics express·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 4, 2026

Measurement of Quantum Interference in a Silicon Ring Resonator Photon Source

Measurement of Quantum Interference in a Silicon Ring Resonator Photon Source

Published on: April 4, 2017

On-chip spectrophotometry for bioanalysis using microring resonators.

Arthur Nitkowski, Antje Baeumner, Michal Lipson

Biomedical Optics Express

|February 23, 2011

Summary

This summary is machine-generated.

This study introduces a microscale optofluidic device for measuring optical absorption in enzymatic reactions. The innovative system achieves high sensitivity for biochemical analysis in small volumes, enabling rapid enzyme activity detection.

Keywords:

(130.6010) Sensors (140.3948) Microcavity devices (170.010) Medical optics and biotechnology (300.6550) Spectroscopy, visible

More Related Videos

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

$Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing$

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

Published on: March 13, 2013

Related Experiment Videos

Last Updated: Jun 4, 2026

Measurement of Quantum Interference in a Silicon Ring Resonator Photon Source

Measurement of Quantum Interference in a Silicon Ring Resonator Photon Source

Published on: April 4, 2017

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

$Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing$

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

Published on: March 13, 2013

Area of Science:

Biochemistry
Optics
Microfluidics

Background:

Biochemical analysis often requires sensitive detection of optical absorption.
Microfluidic devices offer miniaturization and reduced sample consumption for assays.

Purpose of the Study:

To develop and demonstrate a microscale optofluidic device for measuring optical absorption in enzymatic reactions.
To assess the device's sensitivity and performance for biochemical analysis.

Main Methods:

Cavity-enhanced laser spectrophotometry using silicon nitride microring resonators (100 µm radius, Q ~180,000).
Analysis of resonator transmission spectra to determine optical absorption at visible wavelengths within a microfluidic channel.

Main Results:

Achieved a detection limit of 0.12 cm⁻¹ for optical absorption.
Demonstrated detection of individual enzyme activity within minutes in a 100 fL sensing volume.

Conclusions:

Absorption-based microring resonators offer a highly sensitive, compact, and low-analyte-consumption platform.
The developed device shows significant potential for lab-on-a-chip applications in biochemical analysis.