Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbes and the Nitrogen Cycle01:26

Microbes and the Nitrogen Cycle

The nitrogen cycle is a complex biogeochemical process critical to maintaining the balance of nitrogenous compounds in ecosystems. This cycle involves multiple microbial-mediated transformations through which nitrogen changes oxidation states, supporting essential ecological functions and contributing to plant and microbial growth.Nitrogen Fixation and AmmonificationNitrogen fixation initiates the cycle by converting inert atmospheric nitrogen (N₂) into bioavailable ammonia (NH₃), a process...
Microbial Wastewater Treatment01:30

Microbial Wastewater Treatment

Microbial communities in aquatic ecosystems play a key role in the natural breakdown of contaminants introduced through domestic and industrial effluents. Acting as biological catalysts, these microbes change and mineralize a wide range of organic and inorganic pollutants under different redox conditions.In oxygen-rich surface waters, aerobic heterotrophs lead organic matter breakdown, using oxygen as the terminal electron acceptor to efficiently oxidize substrates to carbon dioxide and water.
Overview of Nitrogen Metabolism01:20

Overview of Nitrogen Metabolism

Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of  ammonia, ammonium ions, nitrate, nitrite, or  nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this nitrogen...
Inorganic Nitrogen Assimilation01:22

Inorganic Nitrogen Assimilation

Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme nitrate reductase...
The Nitrogen Cycle01:49

The Nitrogen Cycle

Nitrogen atoms, present in all proteins and DNA, are recycled between abiotic and biotic components of the ecosystem. However, the primary form of nitrogen on Earth is nitrogen gas, which cannot be used by most animals and plants. Thus, nitrogen gas must first be converted into a usable form by nitrogen-fixing bacteria before it can be cycled through other living organisms. The use of nitrogen-containing fertilizers and animal waste products in human agriculture has greatly influenced the...
Bioremediation00:46

Bioremediation

Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Long-term monitoring through a wastewater-based observatory to model urban population dynamics and health indicators.

The Science of the total environment·2026
Same author

Circadian PERIOD proteins regulate TC-DSB repair through anchoring to the nuclear envelope.

Molecular cell·2025
Same author

[Challenges of nutritional care in oncology and hematology for elderly patients].

Soins. Gerontologie·2025
Same author

Removal of pharmaceuticals through UV-C/Performic acid advanced oxidation process: Kinetics and identification of reactive species.

Journal of hazardous materials·2025
Same author

Transcriptional repression facilitates RNA:DNA hybrid accumulation at DNA double-strand breaks.

Nature cell biology·2025
Same author

Towards an online, high-frequency determination of the biochemical methane potential of sewage sludge.

Waste management (New York, N.Y.)·2025
Same journal

Redox-centric metabolic rewiring for dark-fermentative hydrogen production in Enterobacter aerogenes.

Bioprocess and biosystems engineering·2026
Same journal

Improving trehalose production through the design and optimization of linker peptides in scaffold protein.

Bioprocess and biosystems engineering·2026
Same journal

Elicitors derived from endophytic fungus Acremonium sp. enhance triterpenoid and polysaccharide production in the submerged cultivation of the medicinal mushroom Inonotus obliquus.

Bioprocess and biosystems engineering·2026
Same journal

Comparison of three inoculum sources for acetate production and microbial succession in H<sub>2</sub>/CO<sub>2</sub>-fed anaerobic system.

Bioprocess and biosystems engineering·2026
Same journal

Integrated cellulase continuous production and downstream processing using a packed-bed bioreactor for solid-state fermentation by thermophilic fungus.

Bioprocess and biosystems engineering·2026
Same journal

Eco-friendly synthesis of ZnO nanostructures from yeast strains isolated from kombucha and beetroot kwass for antimicrobial thin film applications.

Bioprocess and biosystems engineering·2026
See all related articles

Related Experiment Video

Updated: Jun 4, 2026

Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors
07:59

Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors

Published on: December 6, 2018

Modeling nitrogen removal for a denitrification biofilter.

Guillaume Samie1, Jean Bernier, Vincent Rocher

  • 1Département de génie civil et génie des eaux, Université Laval, 1065 avenue de la Médecine, Quebec, G1V 0A6, Canada.

Bioprocess and Biosystems Engineering
|February 23, 2011
PubMed
Summary
This summary is machine-generated.

Wastewater treatment models can now estimate nitrous oxide (N2O) emissions. This study modified a common model to track N2O production during denitrification, aiding greenhouse gas reduction efforts.

More Related Videos

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
08:05

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
08:13

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities

Published on: December 25, 2015

Related Experiment Videos

Last Updated: Jun 4, 2026

Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors
07:59

Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors

Published on: December 6, 2018

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
08:05

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
08:13

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities

Published on: December 25, 2015

Area of Science:

  • Environmental Engineering
  • Environmental Chemistry
  • Climate Science

Background:

  • Nitrous oxide (N2O) is a potent greenhouse gas contributing to global warming.
  • Wastewater treatment plants (WWTPs) are significant sources of N2O emissions, particularly during nitrification and denitrification.
  • Conventional Activated Sludge Models (ASM) simplify denitrification, failing to quantify intermediate products like N2O.

Purpose of the Study:

  • To modify the ASM1 model to simulate intermediate products of denitrification, including N2O.
  • To estimate N2O emissions from the denitrification stage of a specific WWTP using the enhanced model.
  • To assess the utility of modeling for quantifying N2O emissions in WWTPs.

Main Methods:

  • Implemented a modified ASM1 model within the GPS-X software.
  • Simulated N2O concentrations and emissions over a one-year period for 12 biofilters at the Seine-Centre WWTP.
  • Validated model performance against measured effluent data for nitrate and nitrite.

Main Results:

  • The modified ASM1 model accurately simulated nitrate and nitrite effluent data.
  • Calculated mean N2O emission rate was 4.95 kgN-N2O/day.
  • This calculated value falls within the typical experimental range of 4-31 kgN-N2O/day.

Conclusions:

  • The modified ASM1 model effectively simulates N2O intermediate products and quantifies emissions from WWTP denitrification.
  • Modeling offers a valuable approach for estimating N2O emissions, which are often not directly measured.
  • This approach can support strategies for mitigating greenhouse gas emissions from wastewater treatment processes.