Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

Applications Of NMR In Biology

Applications Of NMR In Biology

Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
The...

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Role of the medial prefrontal cortex in the effects of rapid acting antidepressants on decision-making biases in rodents.

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology·2020

Same author

Growth-control of human ovarian-carcinoma cells by insulin-like growth-factors.

Oncology reports·2011

Same author

Ovarian cancer models : technical review.

Methods in molecular medicine·2011

Same author

Quantitation of FISH Signals in Archival Tumors.

Methods in molecular medicine·2011

Same author

RNase Protection Assay Analysis of mRNA for TGFβ1(-3) in Ovarian Tumors.

Methods in molecular medicine·2011

Same author

Measurement of protein expression a technical overview.

Methods in molecular medicine·2011

Search research articles

Related Experiment Video

Updated: Jun 4, 2026

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

mRNA analyses : a technical overview.

¹Department of Surgery, Glasgow Royal Infirmary, Glasgow, UK.

Methods in Molecular Medicine

|February 23, 2011

Summary

This summary is machine-generated.

This overview highlights fundamental techniques for messenger RNA (mRNA) analysis, covering both qualitative and quantitative methods for gene expression studies in cell lines and tumor biopsies.

More Related Videos

Genomic MRI - a Public Resource for Studying Sequence Patterns within Genomic DNA

Genomic MRI - a Public Resource for Studying Sequence Patterns within Genomic DNA

Published on: May 9, 2011

Related Experiment Videos

Last Updated: Jun 4, 2026

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

Genomic MRI - a Public Resource for Studying Sequence Patterns within Genomic DNA

Genomic MRI - a Public Resource for Studying Sequence Patterns within Genomic DNA

Published on: May 9, 2011

Area of Science:

Molecular Biology
Gene Expression Analysis

Background:

Understanding mRNA analysis techniques is crucial for researchers in molecular biology.
This overview provides foundational knowledge for approaching mRNA analysis.

Purpose of the Study:

To offer a fundamental overview of basic mRNA analysis techniques.
To highlight methods applicable to gene expression studies in cell lines and tumor biopsies.

Main Methods:

Qualitative and quantitative mRNA analysis techniques are discussed.
The overview covers fundamental principles, referencing detailed protocols for differential display, in situ hybridization (PRINS, PCR in situ), and basic polymerase chain reaction (PCR).

Main Results:

Provides researchers with essential understanding for mRNA analysis.
Highlights techniques applicable to diverse biological samples.

Conclusions:

This overview serves as an introductory guide to mRNA analysis techniques.
Researchers can apply these fundamental methods for gene expression studies.