Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

What is Gene Expression?

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Role of the medial prefrontal cortex in the effects of rapid acting antidepressants on decision-making biases in rodents.

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology·2020

Same author

Letters.

The Physician and sportsmedicine·2016

Same author

Growth-control of human ovarian-carcinoma cells by insulin-like growth-factors.

Oncology reports·2011

Same author

Ovarian cancer models : technical review.

Methods in molecular medicine·2011

Same author

Quantitation of FISH Signals in Archival Tumors.

Methods in molecular medicine·2011

Same author

mRNA analyses : a technical overview.

Methods in molecular medicine·2011

Search research articles

Related Experiment Video

Updated: Jun 4, 2026

Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting

Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting

Published on: April 9, 2019

Measurement of protein expression a technical overview.

J R Reeves¹, J M Bartlett

¹Delaware, Ontario, Canada.

Methods in Molecular Medicine

|February 23, 2011

Summary

This summary is machine-generated.

Ovarian cancer, a genetic disease, requires protein analysis for understanding tumor formation. Researchers should validate DNA/RNA findings with protein expression changes to avoid artefactual results.

More Related Videos

High-throughput Protein Expression Generator Using a Microfluidic Platform

High-throughput Protein Expression Generator Using a Microfluidic Platform

Published on: August 23, 2012

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Related Experiment Videos

Last Updated: Jun 4, 2026

Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting

Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting

Published on: April 9, 2019

High-throughput Protein Expression Generator Using a Microfluidic Platform

High-throughput Protein Expression Generator Using a Microfluidic Platform

Published on: August 23, 2012

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Area of Science:

Oncology
Molecular Biology
Genetics

Background:

Ovarian cancer is a genetic disease driven by alterations at the DNA and RNA levels.
Phenotypic changes in cancer result from altered protein expression or function.
Proteins are critical indicators of biological processes and disease states.

Purpose of the Study:

To emphasize the paramount importance of analyzing protein expression in ovarian cancer research.
To guide researchers in validating DNA/RNA findings with corresponding protein level changes.
To address the challenges and perceptions surrounding protein analysis in molecular research.

Main Methods:

Discusses the significance of protein analysis in understanding genetic disease mechanisms.
Highlights the potential for artefactual or coincidental findings when only analyzing DNA/RNA.
Compares the perceived complexity of protein detection with the sensitivity of nucleic acid amplification techniques.

Main Results:

Protein expression analysis is crucial for confirming genetic changes in ovarian cancer.
Discrepancies between DNA/RNA and protein levels warrant critical investigation.
Current protein detection methods may be perceived as less sensitive than nucleic acid-based assays.

Conclusions:

Protein analysis is essential for a comprehensive understanding of ovarian cancer development.
Researchers must correlate molecular findings with protein expression to ensure biological relevance.
Overcoming perceived technical hurdles in protein analysis is vital for advancing ovarian cancer research.