Related Experiment Video
Updated: Jun 4, 2026
08:19
High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology
Published on: May 28, 2020
RT-PCR Quantitation of HSP60 mRNA Expression
R P Perez1, L Pendyala, Z Elakawi
1Section of Hematology/Oncology, Department of Medicine, Darthmouth-Hitchcock Medical Center, Lebanon, NH.
Methods in Molecular Medicine
|February 23, 2011
Summary
Heat shock protein 60 (HSP60) aids protein folding and cellular protection. Its gene expression is linked to cisplatin resistance in ovarian and bladder cancers.
Area of Science:
- Biochemistry
- Molecular Biology
- Oncology
Background:
- Heat shock protein 60 (HSP60) is a chaperonin crucial for protein folding, assembly, and transport within mitochondria.
- HSP60 protects cellular proteins from denaturation during heat stress.
- The HSP60 gene (HSPD1) is highly conserved and its sequence is well-documented.
Purpose of the Study:
- To investigate the role of HSP60 in cancer, specifically its association with cisplatin resistance.
- To quantify HSP60 mRNA expression in preclinical models of human ovarian and bladder carcinomas.
Main Methods:
- Utilized preclinical human ovarian and bladder carcinoma models.
- Quantitated messenger RNA (mRNA) expression levels of HSP60.
Main Results:
- HSP60 gene expression was quantified in preclinical human ovarian and bladder carcinoma models.
- Previous studies indicate a correlation between HSP60 expression and cisplatin resistance.
Conclusions:
- HSP60 plays a significant role in cellular processes, particularly under stress conditions.
- Further research into HSP60's role may elucidate mechanisms of cisplatin resistance in ovarian and bladder cancers.
