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Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
Metastasis02:30

Metastasis

Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...

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Related Experiment Video

Updated: Jun 4, 2026

A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction
10:35

A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction

Published on: April 5, 2018

Detection of MetastaticTumor Cells in Blood by Reverse Transcriptase-Polymerase Chain Reaction.

K Haack1, K Soondrum, M Neumaier

  • 1Medical Clinic, Department of Clinical Chemistry, University Hospital Eppendorf, Hamburg, Germany.

Methods in Molecular Medicine
|February 23, 2011
PubMed
Summary
This summary is machine-generated.

Accurate tumor staging is vital for cancer prognosis. Advanced molecular techniques like polymerase chain reaction (PCR) improve the detection of micrometastasis, enhancing diagnostic sensitivity for better patient outcomes.

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Related Experiment Videos

Last Updated: Jun 4, 2026

A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction
10:35

A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction

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Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
06:53

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Published on: June 8, 2019

Area of Science:

  • Oncology
  • Molecular Diagnostics
  • Cancer Staging

Background:

  • Accurate tumor staging is critical for predicting disease-free interval and patient prognosis in oncology.
  • Conventional histopathology may understage approximately 30% of cancer patients.
  • Immunohistochemical methods using monoclonal antibodies have improved the detection of micrometastasis.

Purpose of the Study:

  • To highlight the advancements in diagnostic sensitivity for detecting micrometastasis.
  • To explain the role of molecular techniques in improving cancer staging and prognosis.

Main Methods:

  • Utilizing nucleic acid amplification and detection methods for enhanced sensitivity.
  • Employing messenger RNA (mRNA) detection assays to identify tumor cells.
  • Reverse transcriptase (RT) to create cDNA from RNA, followed by polymerase chain reaction (PCR) for amplification.

Main Results:

  • Micrometastasis assays achieve significantly increased analytical and diagnostic sensitivity.
  • Detection of specific mRNA molecules distinguishes tumor cells from non-malignant cells.
  • Presence of cytokeratin-positive cells in bone marrow is an independent prognostic marker.

Conclusions:

  • Advanced molecular techniques, particularly RT-PCR, offer superior sensitivity for micrometastasis detection.
  • Improved detection of tumor cells enhances the accuracy of cancer staging and prognostic assessment.
  • These methods hold significant potential for improving patient survival prediction and treatment strategies.