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Updated: Jun 4, 2026
Multi-photon Intracellular Sodium Imaging Combined with UV-mediated Focal Uncaging of Glutamate in CA1 Pyramidal Neurons
Published on: October 8, 2014
Wavelength-selective uncaging of dA and dC residues
Florian Schäfer1, Khashti Ballabh Joshi, Manuela A H Fichte
1Cluster of Excellence Macromolecular Complexes, Goethe University of Frankfurt, Max-von-Laue-Str. 9, 60438 Frankfurt (M), Germany.
Abstract:
Nitrodibenzofuran (NDBF) groups are used as photolabile "caging" groups to temporarily mask the Watson-Crick interaction of dA and dC residues. They show improved masking capabilities and are photodeprotected 12 times more efficiently than 1-(o-nitrophenyl)-ethyl (NPE) caging groups in these positions. Furthermore, NDBF groups can be removed wavelength-selectively in the presence of NPE groups. This will allow more complex (un)caging strategies of oligonucleotides--beyond the usual irreversible triggering.
