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Related Experiment Video

Updated: Jun 4, 2026

Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection
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Label-free multiplexed virus detection using spectral reflectance imaging.

Carlos A Lopez1, George G Daaboul, Rahul S Vedula

  • 1Department of Electrical & Computer Engineering, Boston University, Boston, MA 02215, USA.

Biosensors & Bioelectronics
|February 24, 2011
PubMed
Summary

A new label-free Interferometric Reflectance Imaging Sensor (IRIS) platform enables sensitive detection of whole viruses and viral proteins. This biosensor offers rapid, high-throughput virus identification with minimal sample preparation.

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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Medical Diagnostics

Background:

  • Label-free biosensing platforms are crucial for rapid diagnostics.
  • Interferometric Reflectance Imaging Sensor (IRIS) offers sensitive detection capabilities.
  • Vesicular stomatitis virus (VSV) serves as a model for detecting dangerous viral agents.

Purpose of the Study:

  • To demonstrate a novel label-free platform for whole virus and viral protein detection.
  • To validate the Interferometric Reflectance Imaging Sensor (IRIS) for virus detection.
  • To assess the sensitivity and specificity of the IRIS platform for Vesicular stomatitis virus (VSV).

Main Methods:

  • Utilized spectral reflectance imaging with the Interferometric Reflectance Imaging Sensor (IRIS).
  • Employed surface-immobilized antibodies as capture probes for Vesicular stomatitis virus (VSV).
  • Confirmed detection using fluorescence imaging and tested simultaneous detection of viral proteins.

Main Results:

  • Achieved specific detection of intact Vesicular stomatitis virus (VSV) virions.
  • Established a limit of detection as low as 3.5 × 10^5 plaque-forming units/mL (PFUs/mL).
  • Demonstrated simultaneous detection of external glycoprotein and internal viral proteins in disrupted VSV and cell lysates.

Conclusions:

  • The Interferometric Reflectance Imaging Sensor (IRIS) platform provides sensitive and specific virus detection.
  • Simple surface chemistry and minimal sample preparation are sufficient for effective detection.
  • This label-free quantitative platform holds promise for clinical virus diagnostics.