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Molecular weight determination of underivatized oligodeoxyribonucleotides by positive-ion matrix-assisted ultraviolet
B Spengler1, Y Pan, R J Cotter
1Department of Pharmacology and Molecular Sciences, Middle Atlantic Mass Spectrometry Facility, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Rapid Communications in Mass Spectrometry : RCM
|April 1, 1990
Abstract:
A Wiley-McLaren type time-of-flight mass spectrometer has been used for molecular weight measurements of several unprotected oligodeoxyribonucleotides using matrix-assisted UV laser desorption. Approximately 10 to 100 pmol of sample was required for recording their positive-ion mass spectra with a mass resolution in the range of 150 to 300 (Full width at half maximum) (FWHM). Little fragmentation was observed.