Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 4, 2026

Exploring Independent Effects of Follicle-Stimulating Hormone In Vivo in a Mouse Model

Exploring Independent Effects of Follicle-Stimulating Hormone In Vivo in a Mouse Model

Published on: August 11, 2023

Estrogen-dependent gene expression in the mouse ovary.

Seng H Liew¹, Mai A Sarraj, Ann E Drummond

¹Prince Henry's Institute of Medical Research, Clayton, Melbourne, Australia. Jason.liew@princehenrys.org

|February 25, 2011

Summary

Estrogen influences hundreds of genes in the mouse ovary, impacting reproduction and cellular functions. This study identifies novel estrogen-dependent genes, advancing our understanding of ovarian biology.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Mice lacking NF-ĸB1 undergo premature ovarian aging.

Reproductive biology and endocrinology : RB&E·2026

Same author

Three-Dimensional Models of Implantation Using Human Stem Cells: Scientific Insights and Broader Considerations.

Biomolecules·2025

Same author

Linking Physical Activity to Breast Cancer Risk via Inflammation, Part 1: The Effect of Physical Activity on Inflammation.

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology·2023

Same author

Linking Physical Activity to Breast Cancer via Inflammation, Part 2: The Effect of Inflammation on Breast Cancer Risk.

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology·2023

Same author

Knock down of TIMP-2 by siRNA and CRISPR/Cas9 mediates diverse cellular reprogramming of metastasis and chemosensitivity in ovarian cancer.

Cancer cell international·2022

Same author

Linking Physical Activity to Breast Cancer Risk via the Insulin/Insulin-like Growth Factor Signaling System, Part 2: The Effect of Insulin/Insulin-like Growth Factor Signaling on Breast Cancer Risk.

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology·2022

Area of Science:

Reproductive biology
Endocrinology
Genomics

Background:

Estrogen (E) is crucial for female reproductive system regulation, especially ovarian function.
The full spectrum of estrogen-regulated genes in the ovary is not yet understood.

Purpose of the Study:

To profile estrogen-dependent genes in the mouse ovary using an in vivo model.
To identify novel genes regulated by estrogen in ovarian tissue.

Main Methods:

Utilized wild-type (WT) and aromatase knockout (ArKO; estrogen-free) mouse ovaries.
Analyzed ovarian RNA using microarray (Illumina Sentrix Mouse WG-6 BeadChip, 45,281 transcripts).
Performed comparative analysis to identify differentially expressed genes.

Main Results:

More Related Videos

Ovarian Tissue Culture to Visualize Phenomena in Mouse Ovary

Ovarian Tissue Culture to Visualize Phenomena in Mouse Ovary

Published on: June 19, 2018

Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse

Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse

Published on: September 3, 2021

Related Experiment Videos

Last Updated: Jun 4, 2026

Exploring Independent Effects of Follicle-Stimulating Hormone In Vivo in a Mouse Model

Exploring Independent Effects of Follicle-Stimulating Hormone In Vivo in a Mouse Model

Published on: August 11, 2023

Ovarian Tissue Culture to Visualize Phenomena in Mouse Ovary

Ovarian Tissue Culture to Visualize Phenomena in Mouse Ovary

Published on: June 19, 2018

Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse

Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse

Published on: September 3, 2021

Identified 450 differentially expressed genes influenced by estrogen (291 upregulated, 159 downregulated by ≥2-fold in ArKO vs. WT).
Confirmed previously reported estrogen-regulated genes and discovered novel ones.
Found 78 estrogen-responsive elements (ERE) among genes in key functional categories (hormonal processes, reproduction, sex differentiation, apoptosis, cellular processes).

Conclusions:

Defined the estrogen-regulated transcriptome in the mouse ovary.
This research provides a foundation for further investigation into estrogen's role in follicular development and ovarian function.