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Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids
Published on: June 5, 2015
Induced pluripotent stem cell lines derived from equine fibroblasts
Kristina Nagy1, Hoon-Ki Sung, Puzheng Zhang
1Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, ON, Canada.
Stem Cell Reviews and Reports
|February 25, 2011
Summary
Researchers generated equine induced pluripotent stem (EiPS) cells from horse fibroblasts using a piggyBac transposon system. These EiPS cells show pluripotency and can form teratomas, offering potential for veterinary regenerative medicine and preclinical models.
Area of Science:
- Veterinary Medicine
- Stem Cell Biology
- Regenerative Medicine
Background:
- Domesticated horses are valuable for sports and recreation.
- Horses can serve as models for human medical conditions like musculoskeletal injuries.
- Induced pluripotent stem cells (iPS cells) offer promise for regenerative therapies.
Purpose of the Study:
- To generate equine iPS (EiPS) cells from equine fibroblasts.
- To characterize the pluripotency and stability of the generated EiPS cell lines.
- To explore the potential applications of EiPS cells in veterinary medicine and human preclinical research.
Main Methods:
- Fibroblasts were reprogrammed into iPS cells using a piggyBac transposon system.
- Transgenes delivered reprogramming factors (Oct4, Sox2, Klf4, c-Myc) in a regulated manner.
- EiPS cell lines were assessed for pluripotency markers, karyotype stability, and teratoma formation.
Main Results:
- Successfully generated stable EiPS cell lines from equine fibroblasts.
- EiPS cells expressed key pluripotency markers.
- EiPS cells maintained a stable karyotype during long-term culture.
- In vivo grafting resulted in teratomas containing derivatives of all three embryonic germ layers.
Conclusions:
- Generated EiPS cell lines are pluripotent and stable.
- EiPS cells hold significant promise for developing novel stem cell-based regenerative therapies in veterinary medicine.
- EiPS cells can serve as valuable preclinical models for human applications.
- EiPS cells may offer possibilities for conserving endangered or recently extinct equine species.
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