Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A Case of a 5-Year-Old Boy with a Blauth Type IIIB Hypoplastic Thumb Reconstructed with a Nonvascularized, Hemilongitudinal Metatarsal Transfer.

Case reports in orthopedics·2019

Same author

Therapeutic Targets for Bone and Soft-Tissue Sarcomas.

International journal of molecular sciences·2019

Same author

Risk factors for surgical site infection after malignant bone tumor resection and reconstruction.

BMC cancer·2019

Same author

A Scaffold-Free Allogeneic Construct From Adipose-Derived Stem Cells Regenerates an Osteochondral Defect in a Rabbit Model.

Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association·2019

Same author

The combination of gemcitabine and nab-paclitaxel as a novel effective treatment strategy for undifferentiated soft-tissue sarcoma in a patient-derived orthotopic xenograft (PDOX) nude-mouse model.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2019

Same author

[Development of Novel Osteoporosis Screening Methods Using FRAX].

Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·2019

Search research articles

Related Experiment Video

Updated: Jun 4, 2026

Live Imaging of Drug Responses in the Tumor Microenvironment in Mouse Models of Breast Cancer

Live Imaging of Drug Responses in the Tumor Microenvironment in Mouse Models of Breast Cancer

Published on: March 24, 2013

Tumor imaging with multicolor fluorescent protein expression.

Norio Yamamoto¹, Hiroyuki Tsuchiya, Robert M Hoffman

¹Department of Orthopedic Surgery, Graduate School of Medical Sciences, Kanazawa University, 13-1 Takaramachi, Kanazawa, Ishikawa 920-8641, Japan. norinori@med.kanazawa-u.ac.jp

International Journal of Clinical Oncology

|February 25, 2011

Summary

Fluorescent protein imaging revolutionized in vivo cell biology, enabling real-time visualization of cancer metastasis and treatment efficacy. This technology allows for the study of cancer progression and the development of novel therapeutic strategies.

More Related Videos

Quantitative Multispectral Analysis Following Fluorescent Tissue Transplant for Visualization of Cell Origins, Types, and Interactions

Quantitative Multispectral Analysis Following Fluorescent Tissue Transplant for Visualization of Cell Origins, Types, and Interactions

Published on: September 22, 2013

Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models

Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models

Published on: March 13, 2018

Related Experiment Videos

Last Updated: Jun 4, 2026

Live Imaging of Drug Responses in the Tumor Microenvironment in Mouse Models of Breast Cancer

Live Imaging of Drug Responses in the Tumor Microenvironment in Mouse Models of Breast Cancer

Published on: March 24, 2013

Quantitative Multispectral Analysis Following Fluorescent Tissue Transplant for Visualization of Cell Origins, Types, and Interactions

Quantitative Multispectral Analysis Following Fluorescent Tissue Transplant for Visualization of Cell Origins, Types, and Interactions

Published on: September 22, 2013

Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models

Radionuclide-fluorescence Reporter Gene Imaging to Track Tumor Progression in Rodent Tumor Models

Published on: March 13, 2018

Area of Science:

In vivo cell biology
Cancer research
Medical imaging

Background:

Fluorescent proteins (e.g., GFP, RFP) have transformed biological research.
In vivo imaging allows for real-time observation of cellular processes.
Understanding cancer metastasis and treatment response is crucial.

Purpose of the Study:

To develop and utilize dual-color fluorescent cancer cells for in vivo studies.
To visualize cancer cell dynamics, metastasis, and treatment effects in real time.
To evaluate the efficacy of novel therapeutic interventions.

Main Methods:

Engineered dual-color cancer cells (nucleus-GFP, cytoplasm-RFP) for real-time imaging.
Implanted fluorescently labeled cancer cells in nude mouse models (fibrosarcoma, glioma, pancreatic cancer).
Observed cancer cell trafficking, extravasation, and metastatic colony formation in various organs and lymphatic vessels.
Assessed treatment efficacy of UVC irradiation and Salmonella typhimurium A1-R.

Main Results:

Dual-color cells enabled visualization of nuclear-cytoplasmic dynamics, mitosis, and apoptosis in vivo.
Cancer cell seeding, arrest, accumulation, and colony formation were imaged in real time.
UVC irradiation reduced dual-color cancer cell populations by approximately 70%.
Salmonella typhimurium A1-R significantly increased survival and reduced paralysis in spinal cord glioma models.
Salmonella typhimurium A1-R demonstrated efficacy against primary tumors and metastases in multiple cancer types.

Conclusions:

Fluorescent protein imaging provides unprecedented insights into cancer progression mechanisms.
Dual-color imaging facilitates real-time monitoring of cancer cell behavior and therapeutic responses.
Targeted bacterial therapy shows promise for treating primary tumors and metastases.