[Effect of ascorbic acid for periodontal ligament fibroblast of human deciduous and permanent teeth]

Ascorbic acid (AsA) plays an important role in the formation of collagen in the periodontal ligaments. However, the biochemical role of AsA on the cell metabolism of periodontal ligaments remains undeveloped. This study attempts to explore the effect of AsA and L-Ascorbic acid-2-phosphate (AsA-P) on the cell proliferation and differentiation, and the activity of cell attachment and spreading of conditioned medium (CM) prepared from human fibroblasts derived from the periodontal ligaments of deciduous teeth (HPLF-Y) and permanent teeth (HPLF) according to the explanation methods described by Saito et al. The effect of AsA and AsA-P on DNA synthesis was observed as 15 to 20 percent enhancement in comparison with that of the control for both HPLF-Y and HPLF with exception of AsA-P for HPLF at the 7 day of cell culture. The ALPase activity of HPLF-Y and HPLF at confluent phase was stimulated significantly by the presence of AsA and AsA-P. By morphological observation, the cell spreading activity of the CM of HPLF-Y exposed with AsA-P was higher than that of exposed with AsA. An induction of the cell attachment factors from HPLF-Y is enhanced intensively by the treatment of AsA-P. Therefore, AsA-P may regulate intensively the proliferation and differentiation of HPLF-Y in terms of DNA synthesis and ALPase activity including the enhanced secretion of attachment and spreading factors.