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Updated: Jun 4, 2026

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
Published on: April 21, 2022
[Karyotype analysis of qualified sperm donors on preliminary screening]
Hong-chuan Nie1, Yue-qiu Tan, Guang-xiu Lu
1Institute of Reproduction and Stem Cell Engineering, Human Sperm Bank, Citic-Xiangya Hospital of Reproduction and Genetics, Central South University, Changsha, Hunan 410078, China. niehongchuan@gmail.com
Objective:
To explore the significance of karyotype analysis in screening sperm donors.
Methods:
From January 1, 2004 to December 31, 2008, a total of 2537 potential sperm donors passed our preliminary screening, and all were routinely karyo-typed via peripheral blood. Follow-ups were conducted on the pregnancy outcome and congenital malformation after artificial insemination with the sperm from the qualified donors.
Results:
Among the 2537 qualified sperm donors, 2362 were of the normal karyotype 46, XY and 135 showed polymorphism. Abnormal karyotype was found in 6 cases, and controversial abnormal karyotype in 34.
Conclusion:
Karyotype analysis can reduce the risk of chromosomal disease in neonates from artificial insemination, and genetic counseling for abnormal karyotype sperm donors may help them solve their future reproductive problems.
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