[Cryopreservation of Trichomonas vaginalis and its influence factors]

Different concentrations of dimethylsulfoxide (DMSO) and glycerol, various densities of Trichomonas vaginalis trophozoites and duration of storage were used to study the effects on the cryopreservation of T. vaginalis at -78 degrees C. The optimal densities of trichomonads for cryopreservation were (1-2) x10(6)/ml. Glycerol and DMSO showed a high protective effect, with a maximum effect at a concentration of 10%, and the survival rates were 38.0% and 31.7%, respectively. There was no significant difference between them (P > 0.05). The results showed that the above optimized factors can provide a good protection in short-term (1-16 weeks) cryopreservation, and the trichomonads can be incubated to a logarithmic growth phase at 37 degrees C.