Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A comparative study of methods for determining selenium in biological materials.

J L Burguera1, M Burguera, M Gallignani

  • 1Departamento de Química, Facultad de Ciencias, Universidad de Los Andes, Mérida, Venezuela.

Acta Cientifica Venezolana
|January 1, 1990
PubMed
Summary

This study compared selenium determination methods in biological samples. Fluorimetric and hydride generation atomic absorption spectrometry methods proved most suitable for accurate selenium analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gas exchange and water balance of a mistletoe species and its mangrove hosts.

Oecologia·2017
Same author

Analytical methodology for speciation of arsenic in environmental and biological samples.

Talanta·2008
Same author

On-line cryogenic trapping with microwave heating for the determination and speciation of arsenic by flow injection/hydride generation/atomic absorption spectrometry.

Talanta·2008
Same author

Determination of bismuth in biological samples using on-line flow-injection microwave-assisted mineralization and precipitation/dissolution for electrothermal atomic absorption spectrometry.

Talanta·2008
Same author

Determination of beryllium in natural and waste waters using on-line flow-injection preconcentration by precipitation/dissolution for electrothermal atomic absorption spectrometry.

Talanta·2008
Same author

Sequential determination of Se(IV) and Se(VI) by flow injection-hydride generation-atomic absorption spectrometry with HCl/HBr microwave aided pre-reduction of Se(VI) to Se(IV).

Talanta·2008

Area of Science:

  • Analytical Chemistry
  • Environmental Science
  • Biochemistry

Background:

  • Accurate determination of selenium in biological materials is crucial for health and environmental monitoring.
  • Various analytical techniques exist, but their suitability for complex biological matrices differs.

Purpose of the Study:

  • To comparatively evaluate the analytical performance of multiple methods for selenium determination in biological samples.
  • To identify the most accurate and sensitive techniques for reliable selenium quantification.

Main Methods:

  • Comparative analysis of fluorimetric spectrophotometry, atomic absorption spectrometry (AAS), flow injection analysis with AAS, and hydride generation (HG) with AAS detection.
  • Evaluation based on detection limits, linearity, and sensitivity.

Related Experiment Videos

  • Validation using NBS standard reference materials.

    • Main Results:

    • Fluorimetric spectrophotometry and HG-AAS demonstrated superior detection limits, linearity, and sensitivity.
    • Spectrophotometry, flame AAS, flow injection-AAS, and HG-molecular emission cavity analysis showed inadequate performance.
    • Accuracy of the selected methods was confirmed with standard reference materials.

    Conclusions:

    • Fluorimetric spectrophotometry and HG-AAS are the recommended methods for selenium determination in biological materials.
    • Other evaluated techniques are unsuitable due to inferior analytical performance.
    • Method selection is critical for reliable selenium analysis in complex matrices.