Related Experiment Video

Updated: Jun 4, 2026

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence
04:56

Detection of Aggregation-Prone Behavior in Mutant P53 V157F Breast Cancer Cells Using Multipoint Thioflavin T Fluorescence

Published on: December 30, 2025

FAK and p53 protein interactions

Vita M Golubovskaya1, William G Cance

  • 1Department of Surgical Oncology, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263, USA. Vita.Golubovskaya@roswellpark.org

Anti-Cancer Agents in Medicinal Chemistry
|March 2, 2011
PubMed

Insights

Focal Adhesion Kinase (FAK) and p53 protein interaction regulates cell functions. This review details how FAK and p53 mutually inhibit each other

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Cancer Research

Background:

  • Focal Adhesion Kinase (FAK) is crucial for cell adhesion, motility, and cancer progression.
  • The tumor suppressor protein p53 regulates cell cycle and apoptosis.
  • Previous work established FAK promoter regulation by p53 and direct FAK-p53 interaction.

Purpose of the Study:

  • To review the intricate interaction between FAK and p53 proteins.
  • To elucidate the regulatory feedback loop between FAK and p53.
  • To discuss the molecular mechanisms underlying FAK-p53 cross-regulation.

Main Methods:

  • Cloning of the FAK promoter sequence.
  • Immunoprecipitation, pull-down assays, and confocal microscopy to confirm protein interactions.
  • Identification of the specific amino acid site in p53 involved in FAK interaction.

Main Results:

  • FAK and p53 proteins directly interact within cellular cytoplasm and nucleus.
  • FAK inhibits p53 transcriptional activity on targets like p21, Bax, and Mdm-2.
  • A 7-amino acid site on p53 mediates its interaction with FAK.

Conclusions:

  • The FAK-p53 interaction forms a critical regulatory loop.
  • p53 inhibits FAK promoter activity, while FAK inhibits p53 transcriptional activity.
  • Understanding this interplay is vital for comprehending cell signaling in cancer.

More Related Videos

Yeast As a Chassis for Developing Functional Assays to Study Human P5314:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Purification of Ubiquitinated p53 Proteins from Mammalian Cells10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.