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Related Experiment Video

Updated: Jun 4, 2026

In Utero Intra-cardiac Tomato-lectin Injections on Mouse Embryos to Gauge Renal Blood Flow
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Immunohistochemistry of embryo sections.

Andras Nagy, Marina Gertsenstein, Kristina Vintersten

    CSH Protocols
    |March 2, 2011
    PubMed
    Summary

    This protocol details antigen localization in embryo sections using antibody-based detection. It provides a method for visualizing specific proteins within cells and tissues on glass slides.

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    Area of Science:

    • Cell Biology
    • Histology
    • Immunohistochemistry

    Background:

    • Antigen localization is crucial for understanding cellular function and tissue architecture.
    • Standard methods require precise tissue preparation and antibody-based detection.

    Purpose of the Study:

    • To describe a reliable protocol for antigen localization in embryo sections.
    • To enable visualization of specific antigens within cellular and tissue contexts.

    Main Methods:

    • Utilizes embryo sections mounted on glass slides.
    • Employs alkaline-phosphatase-coupled or horseradish-peroxidase-coupled secondary antibodies for detection.
    • Involves standard immunohistochemical staining techniques.

    Main Results:

    • Successfully demonstrates antigen localization within embryo tissue sections.
    • Provides a reproducible method for visualizing target antigens.

    Conclusions:

    • This protocol offers a straightforward approach for antigen mapping in developmental biology.
    • The method is adaptable for various antigens and detection systems.

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