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Immunohistochemistry of embryo sections

Andras Nagy, Marina Gertsenstein, Kristina Vintersten

    CSH Protocols
    |March 2, 2011
    PubMed

    INTRODUCTIONThis protocol describes how to localize an antigen in cells and tissues using embryo sections attached to glass slides. The method outlined here uses alkaline-phosphatase-coupled secondary antibody; horseradish-peroxidase-coupled secondary antibody can be used as an alternative.

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