Preparation and loading of gelatin nanoparticles

INTRODUCTIONThis protocol describes the synthesis of nanoparticles using the biopolymer gelatin and its derivatives, the loading of DNA to the nanoparticles by encapsulation and adsorption, and the estimation of entrapment efficiency. The steps involved in the preparation of nanoparticles by desolvation using ethanol are presented, as are the precautions to be taken during the encapsulation or adsorption of the payload.