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Related Experiment Video

Updated: May 23, 2026

Linearization of the Bradford Protein Assay
06:35

Linearization of the Bradford Protein Assay

Published on: April 12, 2010

Bradford assay.

Ed Harlow, David Lane

    CSH Protocols
    |April 10, 2012
    PubMed
    Summary
    This summary is machine-generated.

    This protein quantitation assay offers good accuracy for most proteins. However, it is less reliable for small, basic polypeptides and is affected by detergent concentrations above 0.2%.

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    Area of Science:

    • Biochemistry
    • Analytical Chemistry

    Background:

    • Accurate protein quantitation is crucial for various biological and chemical analyses.
    • Existing protein assays may have limitations in specific applications.

    Purpose of the Study:

    • To evaluate the accuracy and limitations of a specific protein quantitation assay.
    • To identify conditions under which the assay's performance is compromised.

    Main Methods:

    • The study assessed the performance of a protein quantitation assay.
    • The assay's accuracy was tested across a range of protein types and conditions.

    Main Results:

    • The assay demonstrated relative accuracy for the majority of proteins analyzed.
    • Exceptions included small basic polypeptides like ribonuclease and lysozyme.

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    Linearization of the Bradford Protein Assay
    06:35

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    Published on: April 12, 2010

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  • Detergent concentrations exceeding approximately 0.2% significantly hampered assay performance.

    • Conclusions:

    • The protein quantitation assay is suitable for general protein measurement but has specific limitations.
    • Researchers should be aware of the assay's reduced accuracy with small basic polypeptides and high detergent levels.