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Intracellular trafficking studies using gold-encapsulated gelatin nanoparticles
CSH Protocols
|March 2, 2011
Abstract:
INTRODUCTIONTrafficking of nanoparticles in cells is best carried out by encapsulation of electron-dense material (e.g., gold) in the nanoparticles and by visualizing them in the cell by transmission electron microscopy (TEM). This protocol describes the preparation of gold particles, their encapsulation in protein nanoparticles, culture conditions, and sample preparation for TEM.

