Protein nanospheres for gene delivery

INTRODUCTIONNanoparticles have been widely used to overcome the barriers for drug delivery. Those prepared from natural polymers have a significant advantage over others prepared from synthetic polymers. This article outlines the advantages of gelatin for the preparation of nanoparticles and a method for preparing them. The uses of nanoparticles are also discussed. Cell trafficking can be studied using nanoparticles encapsulated with electron-dense material (e.g., gold); such particles are then visualized by transmission electron microscopy (TEM). DNA-encapsulated nanoparticles can be used for transfection and other methods of gene delivery. The qualitative and quantitative analysis of transfection studies is outlined briefly.