Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bacterial Transformation01:33

Bacterial Transformation

In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
Bacterial Transformation01:33

Bacterial Transformation

In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Transformation01:26

Transformation

Microbial communities are dynamic environments where cell lysis releases free DNA into the surroundings. Other cells can take up this extracellular DNA through a process known as transformation.When a cell incorporates this foreign DNA into its genome, resulting in genetic modification, the process is known as transformation. Cells capable of this process are termed competent. Competence can be natural, as observed in certain bacteria and archaea, or artificially induced in the...
Source Transformation01:15

Source Transformation

Source transformation is a fundamental technique employed in circuit analysis, offering a valuable tool for simplifying complex electrical circuits. This technique involves the replacement of either a voltage source in series with a resistor by a current source in parallel with a resistor, or vice versa. The key concept here is that when the original sources are deactivated (turned off), the equivalent resistance at the circuit's end terminals remains the same.
It is essential to note that when...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The genomic basis of adaptive leaf variation in the Galápagos giant daisies.

Nature communications·2026
Same author

Transcriptomic analyses reveal common heterophylly regulations in eight amphibious eudicots.

Plant & cell physiology·2026
Same author

Reduced <i>Akr1b7</i> signaling drives ovarian aging and reproductive dysfunction.

iScience·2026
Same author

Chloroplast differentiation in epidermal cells: an environmental response supporting submerged photosynthesis in Rorippa aquatica.

The New phytologist·2026
Same author

Cadmium stress alters heterophylly, anatomy, and photosynthetic activity of Hygrophila difformis.

Ecotoxicology and environmental safety·2025
Same author

CAP peptides artificially induce insect-gall-like growth in different plant species.

Plant & cell physiology·2025
Same journal

Identification of positive GATEWAY expression clones when both the pENTRY and pDEST vectors contain the same marker for bacterial selection.

CSH protocols·2012
Same journal

Imaging protein interactions by FRET microscopy: cell preparation for FRET analysis.

CSH protocols·2012
Same journal

Imaging protein interactions by FRET microscopy: labeling proteins with fluorescent dyes.

CSH protocols·2012
Same journal

Bradford assay.

CSH protocols·2012
Same journal

Detection of ubiquitylated proteins in mammalian cells.

CSH protocols·2012
Same journal

Imaging of organelle membrane systems and membrane traffic in living cells.

CSH protocols·2012
See all related articles

Related Experiment Video

Updated: Jun 4, 2026

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
09:05

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Published on: March 11, 2020

Tomato transformation.

Seisuke Kimura1, Neelima Sinha

  • 1Department of Plant Biology, University of California, Davis, CA 95616, USA.

CSH Protocols
|March 2, 2011
PubMed
Summary
This summary is machine-generated.

This study details a reliable Agrobacterium tumefaciens-mediated transformation protocol for tomato plants. This method enables stable genetic modification for gene function analysis in tomatoes.

More Related Videos

Tomato Analyzer: A Useful Software Application to Collect Accurate and Detailed Morphological and Colorimetric Data from Two-dimensional Objects
15:25

Tomato Analyzer: A Useful Software Application to Collect Accurate and Detailed Morphological and Colorimetric Data from Two-dimensional Objects

Published on: March 16, 2010

An Efficient and Reproducible Method for Producing Composite Plants by Agrobacterium rhizogenes-Based Hairy Root Transformation
04:09

An Efficient and Reproducible Method for Producing Composite Plants by Agrobacterium rhizogenes-Based Hairy Root Transformation

Published on: June 30, 2023

Related Experiment Videos

Last Updated: Jun 4, 2026

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
09:05

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Published on: March 11, 2020

Tomato Analyzer: A Useful Software Application to Collect Accurate and Detailed Morphological and Colorimetric Data from Two-dimensional Objects
15:25

Tomato Analyzer: A Useful Software Application to Collect Accurate and Detailed Morphological and Colorimetric Data from Two-dimensional Objects

Published on: March 16, 2010

An Efficient and Reproducible Method for Producing Composite Plants by Agrobacterium rhizogenes-Based Hairy Root Transformation
04:09

An Efficient and Reproducible Method for Producing Composite Plants by Agrobacterium rhizogenes-Based Hairy Root Transformation

Published on: June 30, 2023

Area of Science:

  • Plant Biology
  • Molecular Biology
  • Genetics

Background:

  • Gene function analysis is crucial for understanding plant biology.
  • Agrobacterium tumefaciens-mediated transformation is a key tool for genetic studies.
  • Efficient transformation protocols are needed for important crop species like tomato.

Purpose of the Study:

  • To describe a robust Agrobacterium-mediated transformation protocol for tomato.
  • To facilitate stable genetic modification of tomato plants.
  • To support research on gene function in tomato.

Main Methods:

  • Utilizing Agrobacterium tumefaciens for T-DNA transfer.
  • Implementing a specific protocol for tomato transformation.
  • Focusing on stable integration of foreign DNA.

Main Results:

  • Successful stable transformation of tomato using the described method.
  • Demonstration of Agrobacterium-mediated T-DNA transfer in tomato.
  • Establishment of a reproducible transformation protocol.

Conclusions:

  • The presented protocol provides an effective means for tomato transformation.
  • This method is valuable for functional genomics research in tomato.
  • Agrobacterium-mediated transformation remains a powerful tool for crop improvement and research.