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Related Experiment Video

Updated: Jun 4, 2026

Bone Marrow-derived Macrophage Production
07:06

Bone Marrow-derived Macrophage Production

Published on: November 22, 2013

Bone Marrow-Derived Macrophages (BMM): Isolation and Applications.

Joachim Weischenfeldt1, Bo Porse

  • 1The Biotech Research and Innovation Centre, University of Copenhagen, 2200 Copenhagen, Denmark.

CSH Protocols
|March 2, 2011
PubMed
Summary

This study details a protocol for generating bone marrow-derived macrophages (BMMs) using macrophage colony-stimulating factor (M-CSF). The method ensures high differentiation efficiency and provides techniques for assessing phagocytic activity and gene delivery in these crucial immune cells.

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Area of Science:

  • Immunology
  • Cell Biology
  • Biotechnology

Background:

  • Bone marrow-derived macrophages (BMMs) are critical immune cells essential for host defense.
  • Macrophage colony-stimulating factor (M-CSF) drives the proliferation and differentiation of myeloid progenitors into macrophages.
  • M-CSF deficiency leads to severe immune defects, including a lack of macrophages and osteoclasts.

Purpose of the Study:

  • To establish a reliable protocol for in vitro differentiation of bone marrow cells into mature BMMs.
  • To provide methods for assessing BMM differentiation efficiency, phagocytic capacity, and amenability to genetic manipulation.
  • To enable further research into macrophage biology and function.

Main Methods:

  • Bone marrow cells cultured with M-CSF from L929-conditioned medium.

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Last Updated: Jun 4, 2026

Bone Marrow-derived Macrophage Production
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Bone Marrow-derived Macrophage Production

Published on: November 22, 2013

Isolation and Culture of Bone Marrow-Derived Macrophages from Mice
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Isolation and Culture of Bone Marrow-Derived Macrophages from Mice

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Isolation and In vitro Culture of Bone Marrow-Derived Macrophages for the Study of NO-Redox Biology
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Isolation and In vitro Culture of Bone Marrow-Derived Macrophages for the Study of NO-Redox Biology

Published on: May 31, 2022

  • Fluorescence-activated cell sorting (FACS) using Mac-1 and 4/80 surface antigens to assess differentiation.
  • Phagocytic efficiency assay using fluorescently labeled yeast zymosan bioparticles.
  • DNA and small interfering RNA (siRNA) delivery methods for BMMs.
  • Carboxyfluorescein succinimidyl ester (CFSE) assay for proliferation analysis.

    • Main Results:

    • A homogenous population of mature BMMs was successfully generated.
    • Differentiation efficiency was confirmed via FACS analysis.
    • Methods for assessing phagocytosis, gene delivery, and proliferation were established and validated.

    Conclusions:

    • The described protocol provides a robust method for generating and characterizing bone marrow-derived macrophages.
    • These differentiated BMMs are suitable for diverse experimental applications in immunology and cell biology.
    • The protocol facilitates studies on macrophage function, including phagocytosis and genetic manipulation.