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Related Experiment Video

Updated: Jun 4, 2026

Assays for Validating Histone Acetyltransferase Inhibitors
09:11

Assays for Validating Histone Acetyltransferase Inhibitors

Published on: August 6, 2020

In vitro histone methyltransferase assay.

Ian M Fingerman1, Hai-Ning Du, Scott D Briggs

  • 1Department of Biochemistry and Purdue Cancer Center, Purdue University, West Lafayette, IN 47907-2064, USA.

CSH Protocols
|March 2, 2011
PubMed
Summary

This study presents a simple in vitro assay to measure histone methyltransferase activity using bacterial cell extracts. This method allows for rapid characterization of enzyme function and substrate specificity without extensive purification.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Epigenetics

Background:

  • Histone methyltransferases (HMTs) are crucial enzymes involved in epigenetic regulation.
  • Dysregulation of HMTs is implicated in various cancers and diseases.
  • Many putative HMTs lack functional characterization.

Purpose of the Study:

  • To develop an efficient in vitro assay for characterizing histone methyltransferase activity.
  • To enable functional analysis of newly identified HMTs.
  • To facilitate pilot studies and substrate specificity determination.

Main Methods:

  • Utilizes bacterial cell extracts expressing the HMT of interest.
  • Incubates extracts with S-adenosyl-L-[methyl-(3H]-methionine and histone substrates.

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Last Updated: Jun 4, 2026

Assays for Validating Histone Acetyltransferase Inhibitors
09:11

Assays for Validating Histone Acetyltransferase Inhibitors

Published on: August 6, 2020

In vitro Methylation Assay to Study Protein Arginine Methylation
10:01

In vitro Methylation Assay to Study Protein Arginine Methylation

Published on: October 5, 2014

Antibody-Free Assay for RNA Methyltransferase Activity Analysis
08:31

Antibody-Free Assay for RNA Methyltransferase Activity Analysis

Published on: July 9, 2019

  • Measures methyl group incorporation via scintillation counting and SDS-PAGE/fluorography.

    • Main Results:

    • The assay allows for direct measurement of HMT activity.
    • Substrate specificity can be readily determined.
    • Enzyme purification is often unnecessary, simplifying the process.

    Conclusions:

    • This protocol provides a robust and accessible method for HMT characterization.
    • The assay is suitable for initial functional screening of HMTs.
    • Facilitates research into the role of HMTs in health and disease.